Think you’ve got your head wrapped around Biotechnology? Put your knowledge to
the test. Good luck — the Stickman is counting on you!
Q. Restriction fragments can be separated from each other based on size using
Electrophoresis
Restriction enzyme digestion
PCR
RT-PCR
Southern blotting
Q. How can a scientist ensure that all of the bacteria they are growing contain the recombinant DNA they have introduced?
Incubate the bacteria at a high temperature
Incubate the bacteria at a low temperature
Incubate the bacteria with the same antibiotic for which the recombinant DNA expresses a resistance gene
Incubate the bacteria for a short period of time
Incubate the bacteria for a long period of time
Q. Why do cloning vectors contain genes for antibiotic resistance in addition to target genes?
Antibiotics can be applied to select for the cells containing the plasmid
Antibiotic resistance genes are the only ones successfully transferred to GM foods
The cloning vectors will not work without antibiotic resistance genes
The bacteria are toxic to plants and have to be killed before the genes can be transferred to plants
Antibiotic resistance genes are more cost effective to use than the alternatives
Q. DNA microarray analysis would be useful in which of the following situations?
To determine the length of a DNA fragment
To determine the sequence of a piece of DNA
To identify a particular DNA sequence
To identify a particular DNA mutation
To look at global changes in gene expression
Q. The step of PCR that has to occur prior to primers binding to the DNA is
Denaturation
Annealing
Extension
Propagation
Filtration
Q. RNAi requires which of the following?
Primers
DNA probes
RNA polymerase
siRNAs
siDNAs
Q. A farmer has a crop of papaya that are being killed by a virus. How could genetic modification of the papaya plants be beneficial?
It would not be beneficial in this scenario
Genes that encode for viral resistance could be transferred to the papaya plant
Viruses that infect papaya will not grow in genetically modified plants
Genetic engineering of papaya plants is only successful for viral resistance
Genes for faster growth could be transferred to the papaya plant
Q. How are seedless watermelons, which are modified using chemicals, a type of genetic modification?
The genome is modified to have fewer chromosomes than necessary, which silences genes for seed production
Seeds are treated with chemicals producing a tetraploid plant that is sterile when crossed with other tetraploids
Seeds are treated with chemicals creating a tetraploid plant that is crossed with a diploid plant, creating a sterile triploid
Seeds from seedless watermelons are collected to create chemically modified foods
A gene is added to inhibit seed growth
Q. A condition that is caused by a gene of known sequence can be diagnosed by
DNA microarray analysis
Western blotting
In vitro mutagenesis
PCR analysis
All of the above
Q. Which of the following is not a problem associated with gene therapy?
Cloning a gene into a viral vector is met with little success
The effect is not long lasting
Gene therapy may activate an immune response
Diseases can be the result of more than one gene
Viral vectors can be toxic to human cells